Search results for "dark fermentation"
showing 9 items of 9 documents
Thermophilic hydrogen production from cellulose with rumen fluid enrichment cultures: Effects of different heat treatments
2011
Elevated temperatures (52, 60 and 65 °C) were used to enrich hydrogen producers on cellulose from cow rumen fluid. Methanogens were inhibited with two different heat treatments. Hydrogen production was considerable at 60 °C with the highest H2 yield of 0.44 mol-H2 mol-hexose -1 (1.93 mol-H2 mol-hexose-degraded-1) as obtained without heat treatment and with acetate and ethanol as the main fermentation products. H2 production rates and yields were controlled by cellulose degradation that was at the highest 21%. The optimum temperature and pH for H2 production of the rumen fluid enrichment culture were 62 °C and 7.3, respectively. The enrichments at 52 and 60 °C contained mainly bacteria from …
Alkaline pretreatment to enhance one-stage CH4 and two-stage H2/CH4 production from sunflower stalks: Mass, energy and economical balances
2015
Abstract Biological production of second generation biofuels such as biohydrogen (H2) or methane (CH4) represents a promising alternative to fossils fuels. Alkaline pretreatments of lignocellulosic biomass are known to enhance the accessibility and the bioconversion of hollocelluloses during anaerobic digestion and dark fermentation processes. In the present study, four different configurations were investigated: one-stage CH4 continuous and two-stage H2 batch/CH4 continuous process with and without alkaline pretreatment of sunflower stalks (55 °C, 24 h, 4 g NaOH/100 g TS). The results showed that two stage H2/CH4 (150 ± 3.5 mL CH4 g−1 VS) did not improve methane yields compared to one stag…
Hydrogen Dark Fermentation for Degradation of Solid andLiquid Food Waste
2021
The constant increase in the amount of food waste accumulating in landfills and discharged into the water reservoirs causes environment pollution and threatens human health. Solid and liquid food wastes include fruit, vegetable, and meat residues, alcohol bard, and sewage from various food enterprises. These products contain high concentrations of biodegradable organic compounds and represent an inexpensive and renewable substrate for the hydrogen fermentation. The goal of the work was to study the efficiency of hydrogen obtaining and decomposition of solid and liquid food waste via fermentation by granular microbial preparation (GMP). The application of GMP improved the efficiency of the d…
Co-production of Hydrogen and Methane From the Organic Fraction of Municipal Solid Waste in a Pilot Scale Dark Fermenter and Methanogenic Biofilm Rea…
2018
The co-production of biohydrogen and methane from the organic fraction of municipal solid waste was investigated using a two-stage AD system, composed of a pilot scale dark fermenter (DF) and a continuous methanogenic biofilm reactor. From the DF process, a biohydrogen yield of 41.7 (± 2.3) ml H2/gVSadded was achieved. The liquid DF effluent (DFE) was rich in short chain volatile fatty acids, i.e., mainly acetic and butyric acid. The DFE was valorized by producing methane in the methanogenic biofilm reactor. Two methanogenic biofilm reactors were used to assess the biotic and abiotic role of the DFE on the performance of the reactors. Regardless of the different DFE feeding (i.e., biotic an…
Effects of heat treatment on hydrogen production potential and microbial community of thermophilic compost enrichment cultures
2011
Cellulosic plant and waste materials are potential resources for fermentative hydrogen production. In this study, hydrogen producing, cellulolytic cultures were enriched from compost material at 52, 60 and 70°C. Highest cellulose degradation and highest H(2) yield were 57% and 1.4 mol-H(2) mol-hexose(-1) (2.4 mol-H(2) mol-hexose-degraded(-1)), respectively, obtained at 52°C with the heat-treated (80°C for 20 min) enrichment culture. Heat-treatments as well as the sequential enrichments decreased the diversity of microbial communities. The enrichments contained mainly bacteria from families Thermoanaerobacteriaceae and Clostridiaceae, from which a bacterium closely related to Thermoanaerobiu…
One-stage H2 and CH4 and two-stage H2+ CH4 production from grass silage and from solid and liquid fractions of NaOH pre-treated grass silage
2009
Abstract In the present study, mesophilic CH4 production from grass silage in a one-stage process was compared with the combined thermophilic H2 and mesophilic CH4 production in a two-stage process. In addition, solid and liquid fractions separated from NaOH pre-treated grass silage were also used as substrates. Results showed that higher CH4 yield was obtained from grass silage in a two-stage process (467 ml g−1 volatile solids (VS)original) compared with a one-stage process (431 ml g−1 VSoriginal). Similarly, CH4 yield from solid fraction increased from 252 to 413 ml g−1 VSoriginal whereas CH4 yield from liquid fraction decreased from 82 to 60 ml g−1 VSoriginal in a two-stage compared to …
Spatial Succession for Degradation of Solid Multicomponent Food Waste and Purification of Toxic Leachate with the Obtaining of Biohydrogen and Biomet…
2022
A huge amount of organic waste is generated annually around the globe. The main sources of solid and liquid organic waste are municipalities and canning and food industries. Most of it is disposed of in an environmentally unfriendly way since none of the modern recycling technologies can cope with such immense volumes of waste. Microbiological and biotechnological approaches are extremely promising for solving this environmental problem. Moreover, organic waste can serve as the substrate to obtain alternative energy, such as biohydrogen (H2) and biomethane (CH4). This work aimed to design and test new technology for the degradation of food waste, coupled with biohydrogen and biomethane prod…
Unexpected high production of biohydrogen from the endogenous fermentation of grape must deposits
2020
International audience; The aim of this work was to assess the performances of wine byproduct biomass for hydrogen production by dark fermentation. Grape must deposits from two grape varieties (Pinot Gris and Chardonnay) were considered, either with external microbial inoculum or without. We show that grape must residues contain endogenous microflora, well adapted to their environment, which can degrade sugars (initially contained in the biomass) to hydrogen without any nutrient addition. Indeed, hydrogen production during endogenous fermentation is as efficient as with an external heat-treated inoculum (2.5 ± 0.4 LH2.L-1reactor and 1.61 ± 0.41 molH2.mol-1consumed hexose, respectively) with…
Metal hydride alloys for storing hydrogen produced by anaerobic bacterial fermentation
2016
Abstract This study reports on hydrogen sorption from bacterial fermentation media with powdered palladium (Pd) and alloys (LaNi5, AB5, and AB2) that are capable of forming hydrides. Mass changes of the powders after incubation in fermentation media were measured by differential thermogravimetry. Composition and concentrations of the gases accumulated during fermentation and absorbed by Pd or the alloys were analyzed by mass spectrometry. The results demonstrated that hydrogen (H2) was absorbed and stored by powdered Pd and alloys directly from nutritional broth. The best sorption was obtained with Pd, followed by alloys AB5 and AB2. Scanning electron microscopy revealed that bacteria were …